The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 1. When connecting for the first time to either the SpectraMax L reader or StakMax system. View the Molecular Devices website. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. APP Automated. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. SoftMax® Pro 7. The. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. Read More. 1-2 sec. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial Rack Bottom Label 2D Reader) Microscan: MS-3 Laser Bar Code Reader: Thermo Fisher Scientific: VisionMate 96 (Vial Rack Bottom Label 2D Reader). Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 6") Weight. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. This offer includes SpectraMax i3x, SpectraMax. Turn on Plate Reader and wait for it to complete its start-up routine. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. You can also contact Technical Support by email. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Plate reader instructions Computer. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Read height should be optimized forVmax® plate reader specifications. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Shortside barcode reader: 0310-5102. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. For more sample throughput, both readers can be easily. absorbance plate readers. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Here, we describe a novel GCaMP6s-CAAX-based calcium assay utilizing a high-throughput fluorometric imaging plate reader (Molecular Devices, Sunnyvale, CA) format that can identify both activators and inhibitors of T-type Ca 2+ channels. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Restart the system. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. IMAP assays are based on binding of. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The sample is typically placed in a cuvette through which light is sent horizontally. Three low volume 24-well bottom slides. Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. 314 low lamp level warning. 더 많은. Page 28 Chapter 5: Read Modes and Read Types 1. . Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. This can increase throughput and efficiency, thus saving time and space in the lab. Find our technical support information along with contact details. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). This document is provided to customers who have purchased Molecular Devices, Inc. The Back Panel Figure 1. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Microplate Reader Shelf. #SpectraMax L Config). The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. For more sample throughput, the system can be easily integrated into a full robotic to that of dedicated single-mode readers. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. 58×38×22 cm (22. These systems are hybrids that allow you to choose between. S. It features a modular design that allows users to add or change detection cartridges according to their needs. Other features and benefits include: Eliminate changing out filters. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Cellular. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Every step is optimized to simplify analysis and reporting to support our microplate readers. Test up to 3. Automated microplate readers reduce manual manipulation and ensure consistent operation. We would like to show you a description here but the site won’t allow us. Description Figure 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Experimental details and customization of the group table. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Instrument settings can be saved as a protocol file. Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. Fluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. (Figure 1). Versa Max. 9000-0756. 1. StakMax Handling Stacker Base Unit. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesMolecular Devices Software. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. A). The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 4: Test tube cover. +1 800-635-5577. 1. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Lamp source is a Xenon flash lamp (1 Joule/flash). This process should take about 3 - 5 minutes. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. 0) OD (500 to 1100) RFU (5. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory solution to help scientists increase. 00. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Avoid losing high signal. The EMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. Ratios for each sample were calculated as Read B/Read A. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Versa. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Data collection speed is sufficient for running fast cell-based kinetic assays. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. The plate reader optics are tunable for absorbance. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Multimode Microplate Readers. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Also see: SoftMax® Pro Microplate Software User Manual Download Page. Molecular Devices technical support is available by phone and e-mail. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. BMG Labtech - THERMOstar. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 2. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may be SoftMax Pro GxP Software. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Check software compatibility with Windows 10 and locate your software installer. +44-118-944-8000. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. The system features 96, 384, and 1536 simultaneous liquid transfer, multi-wavelength kinetic reading, fluorescence and luminescence detection, agile internal plate handling, and elevated instrument0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. View SoftMax Pro GxP. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Molecular Devices. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Longside barcode reader: 0310-5101. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. W×D×H. 1. SoftMax Pro Software. White plates reflect the light, enabling even low levels of Luminescence to be detected. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Flexibility to read a variety of plate formats. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on. Measure more accurately. Read height should be optimized for Vmax® plate reader specifications. The plate is set up for reading and images are acquired according to specified parameters. Table of Contents. Molecular Devices SpectraMax Gemini XS microplate reader with laptop and software (Pre-owned) $5,500. A preconfigured. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. The computer controlling the plate reader runs Windows XP. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software. S. Create custom fluorescence assays without having to purchase expensive filter sets. The plate reader optics are tunable for absorbance,. FlexStation® 3 Multi-Mode Microplate Reader. 1700 +1 800. Select the Driver tab. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The plate reader temperature was set to 37°C and allowed to reach 37°C before proceeding. To update the driver. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. Plus 384. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. With optimized reagents, option to operate the. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1 software Download page Molecular Devices Software SoftMax® Pro. Also refer to the Multimode Reader Comparison Chart Features. Step. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . 340 PC384. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. We would like to show you a description here but the site won’t allow us. The homogeneous Neurotransmitter Transporter Uptake Assay Kit from Molecular Devices Corporation provides a fast, simple and reliable fluorescence-based. 0 EU/mL, but the additional standard concentration (0. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Supplier: Molecular Devices. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. This reader's dual monochromators for. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. in steps of 0. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. 6 kg (30 lbs. gene expression with a user-friendly reader. The assay can be performed on any bottom-read mode fluorescence. Microplate Stacker and Washer. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. For SpectraMax ABS Plus reader, r 2 = 0. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 2. 190. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. It may be named "Pothos". What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Figure 9. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 76358-624. Plate Formats 6 to 1536 wells. The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. , call 1-800-635-5577. 9. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. FlexIII. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. in steps of 0. The assay is easy to set up and the range of potential analytes is vast, but the assay procedure is time consuming and labor intensive. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. Feb 22, 2023The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. With the discontinuous kinetics feature in SoftMax Pro Software. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 1. Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. Clone Screening. 8×15×8. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. QuickSync. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. GxP regulated industry assessments of microplate readers. Microscopes and Microplate Instrumentation. Instrument settings can be saved as a protocol file. Read times. Upgradeable modules are also available. Figure 1. Wait 20 seconds. Wait 20 seconds. Uncontrolled Copy for Training and Reference Purposes Only . One plate carrier adapter is provided with the instrument. Molecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. It also allows for the elimination of standard curves. These systems are hybrids that allow you to choose between. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Step 4: Incubate at room temperature for 1 hour, protected from light. 0 . FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Published Dec 16, 2020 | Updated Dec 16, 2020. The Versamax Microplate Reader is built around a grating monochromator. 1. Molecular Devices' microplate readers. 9000-0756. 1. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Label-free detection. 0. PURPOSE. Turn the reader on and secure the USB cable. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. PURPOSE. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. please click here. Find new and used lab equipment and shop a wide selection of products and vendors. Supersedes 1. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. VIS absorbance detection. 5 µL of each sample was pipetted into a 96-well. When the user applies a set temperature of 37°C, the top is heated to 37. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. Recertification service. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. These lab workhorses are used in drug discovery. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. , June 1, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of FLIPRTETRA the first system that enables researchers to significantly increase throughput of cell-based assays by measuring 1536 kinetic measurements simultaneously. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Unplug the power cable from the back of the instrument. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. W×D×H. Extended linear dynamic range. Plate. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Molecular Devices Software. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. The ultimate guide to microplate readers is an eBook that tells you everything you need to know. Superior optics Microplate Reader Shelf. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. . To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. #4TI-0655) Microplates. 0. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 2. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Temperature Control 4°C above ambient to 45°C. SpectraMax i3x. 3. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. With SoftMax Pro Software, the PathCheck Sensor automatically normalizesThe SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Step 2: Launch SoftMax Pro Software. 2. begin reading microplates with seven mouse clicks. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The Synergy H4 is a convenient benchtop microplate reader that offers robust. V. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Endpoint. In addition, the iD5 reader measures TRF and FP and. The adapter is required for optimum performa nce with standard 96-well and 384-. * Note: Programming the washer with a final aspirate height of 4. With various on-board features and. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. Multi-mode Plate Reader. Load up the plates that you need to run and walk. Weight. Next generation SpectraMax® absorbance microplate readers. Microplate Readers. EMax. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 13. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Plate section without PathCheck applied. First, as mentioned before, the walkaway time significantly.